Each respective difficulty becomes harder and harder as the player progresses through them, making the rate of infectivity to their respective plague a greater challenge to pass on in-game, and are at a higher risk for early detection of a plague. The game has four Difficulty levels: Casual, Normal, Brutal, and Mega-Brutal. Time trials, known in-game as Speed Run mode, are also applicable to the game, as these provide how fast the player can affect the world with their plague and whether they are able to beat their previous record if already done so.
These contain certain events that may affect the normal functionality of the world, or present a simulation of a real-life disease that has been previously researched or occurred at some point in time (i.e. Scenarios, another DLC that includes a lot of exclusive gaming material, is also available to the player. The remaining four are special plagues, or add-ons/DLCs, which are the Neurax Worm, Necroa Virus, Simian Flu, and the Shadow Plague.
These are, in order of unlocking, Bacteria, Virus, Fungus, Parasite, Prion, Nano-Virus, and Bio-Weapon. Seven of these are the standard plague types, which the player does not have to pay for. There are, currently, a total of eleven basic forms of plagues available to the player. The game holds various forms of diseases, each with their unique traits and capabilities regarding rate of infection, durability in different climates, and transmission.
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